Read e-book online Confocal scanning optical microscopy and related imaging PDF

By Gordon S. Kino, Timothy R. Corle

ISBN-10: 0124087507

ISBN-13: 9780124087507

This publication presents a accomplished advent to the sector of scanning optical microscopy for scientists and engineers. The ebook concentrates in most cases on tools: the Confocal Scanning Optical Microscope (CSOM), and the Optical Interference Microscope (OIM). A accomplished dialogue of the idea and layout of the Near-Field Scanning Optical Microscope (NSOM) is additionally given. The textual content discusses the sensible points of creating a confocal scanning optical microscope or optical interference microscope, and the purposes of those microscopes to part imaging, organic imaging, and semiconductor inspection and metrology.A complete theoretical dialogue of the intensity and transverse answer is given with emphasis put on the sensible result of the theoretical calculations and the way those can be utilized to assist comprehend the operation of those microscopes. Key gains * offers a accomplished creation to the sector of scanning optical microscopy for scientists and engineers * Explains many sensible purposes of scanning optical and interference microscopy in such different fields as biology and semiconductor metrology * Discusses in theoretical phrases the starting place of the enhanced intensity and transverse answer of scanning optical and interference microscopes with emphasis at the useful result of the theoretical calculations * Considers the sensible elements of establishing a confocal scanning or interference microscope and explores the various layout tradeoffs made for microscopes utilized in a number of functions * Discusses the speculation and layout of near-field optical microscopes * Explains section imaging within the scanning optical and interference microscopes

Show description

Read or Download Confocal scanning optical microscopy and related imaging systems PDF

Similar imaging systems books

Advanced Signal Processing Handbook: Theory and - download pdf or read online

Advances in electronic sign processing algorithms and desktop expertise have mixed to supply real-time platforms with functions some distance past these of simply few years in the past. Nonlinear, adaptive equipment for sign processing have emerged to supply larger array achieve functionality, even if, they lack the robustness of traditional algorithms.

Heung-Yeung Shum's Image-Based Rendering PDF

Image-based rendering (IBR) refers to a set of strategies and representations that permits 3D scenes and gadgets to be visualized in a pragmatic approach with no complete 3D version reconstruction. the opportunity of photorealistic visualization has large allure, in particular for purposes reminiscent of games, digital shuttle, and E-commerce, which stand to vastly make the most of this expertise.

Download e-book for iPad: Stochastic Image Processing by Chee Sun Won, Robert M. Gray (auth.)

Stochastic photograph Processing offers the 1st thorough remedy of Markov and hidden Markov random fields and their program to picture processing. even supposing promoted as a promising strategy for over thirty years, it has purely been some time past few years that the speculation and algorithms have constructed to the purpose of delivering invaluable ideas to previous and new difficulties in photograph processing.

Download e-book for kindle: Introduction to image stabilization by Restaino, Sergio R.; Teare, Scott W

Using photo stabilization has grown to the purpose that it really is now a typical section of smooth optical platforms for imaging, communications, and remote-sensing functions. the advantages of snapshot stabilization to astronomical examine by myself are so wealthy that it's normal for astronomical telescopes, outfitted during the last century, to be retrofitted with speedy steerage mirrors and tip-tilt sensors to increase their valuable lifetimes.

Extra resources for Confocal scanning optical microscopy and related imaging systems

Example text

A fixed or rotating analyzer is then used in front of the eyepiece to block light with the original polarization. Phase Contrast Imaging The techniques described above rely on intensity changes caused by a change in transmission, reflectivity, fluorescence, or scattering to form an image. An alternative method is to image the phase rather than the amplitude of the transmitted or reflected light. 17 A simplified schematic of a Zernike phase microscope is shown in Fig. 18. ~8 In Zernike phase contrast microscopy the object is illuminated by an annular source, and a phase plate is positioned in the back focal plane of the objective lens.

Speckle occurs because coherent light, reflected from different points of an optically rough surface (rough on the scale of the wavelength of light), adds in or out of phase at the detector. These individual bright speckles are formed in regions where the amplitudes add and have dimensions of the order of the focal spot size dr(3 dB). The speckly appearance of an image formed with laser illumination is unfamiliar and unpleasant, since we are used to viewing images in incoherent light. The actual amplitude and appearance of the speckles depend on how the sidelobe amplitudes from the randomly located reflectors add.

Because the PSF of the CSOM falls off more rapidly than that of the standard microscope, its edge response is also sharper. As expected, the 50% intensity point for the standard microscope corresponds to the 25% intensity point in a CSOM. The formula for the edge response is given in Chapter 3. Transfer Function The amplitude response Ac(r)= hZ(r) of the CSOM dictates the nature of the image. Since this amplitude response is identical to the intensity response of a standard microscope; it follows that the CTF of the CSOM is identical to the OTF of the standard microscope; that is, it has the same triangular shape as shown in Fig.

Download PDF sample

Confocal scanning optical microscopy and related imaging systems by Gordon S. Kino, Timothy R. Corle


by Mark
4.5

Rated 4.87 of 5 – based on 18 votes