By James A. Peters, Braulio Orejas-Miranda, Roberto Donoso-Barros
Pp. (6), 347, 25, (v-viii), 293; a hundred+ text-figures (line-drawings). unique eco-friendly stiff wrappers, lettered in white at the backbone and entrance hide, quarto. half I provides the snakes and half II covers Lizards and Amphisbaenians. this can be a revised variation of the unique half set issued in 1970 as usa nationwide Museum Bulletin, no. 297. New fabric has been extra to either elements via P. E. Vanzolini. exact keys are supplied to all species. No possession marks and no indicators of use.
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Extra resources for Catalogue of the neotropical Squamata: Part 2, lizards and amphisbaenians
The homogenate is centrifuged at 20,000 g for 10 min and fractionated by the addition of solid ammonium sulfate between 50 and 90% saturation, keeping the pH near 8 by the dropwise addition of ammonia. The dark red precipitate is collected by centrifugation, taken up in 1-2 volumes of buffer, and again fractionated with ammonium 53 A. Rossi Fanelli and E. Antonini, Biokhimiya 22, 336 (1957). 54 B. A. Wittenberg, R. W. Briehl, and J. B. Wittenberg, Biochem. J. 96, 363 (1965). 55 L. Tentori, G. Vivaldi, S.
Sephadex and cellulose have very different physical properties. Columns of cellulose derivatives are denser than those of Sephadex, and with some systems the hemoglobin components are eluted in more compact zones. Sephadex columns are spongy and very sensitive to pressure and flow rate. A common procedure has been to use CM-Sephadex or CM-cellulose with a positive pH gradient. Since the starting pH is usually < 7, chromatography with HbO2 results, as discussed earlier, in much methemoglobin and the formation of spurious chromatographic fractions.
Kunz, and K. J. Wilson, Eur. J. Biochem. 88, 387 (1978). 76 D. Keilin and J. F. Ryley, Nature (London172, 451 (1953). r7 M. H. Smith, P. George, and J. R. Preer, Arch. Biochem. Biophys. 99, 313 (1962). 7a E. Steers and R. H. Davis, Comp. Biochem. Physiol. 6211, 393 (1979). 79 R. Oshino, J. Asakura, K. Takio, N. Oshino, and B. Chance, Eur. J. Biochem. 39, 581 (1973).  ERYTHROCRUORINS AND CHLOROCRUORINS  P r e p a r a t i o n By of High Molecular Hemoglobins 43 Weight Invertebrate JOSEPH BONAVENTURA a n d CELIA BONAVENTURA The hemoglobins of the invertebrates are responsible for essentially the same function as those of the vertebrates: carrying oxygen from the environment to the respiring tissues.
Catalogue of the neotropical Squamata: Part 2, lizards and amphisbaenians by James A. Peters, Braulio Orejas-Miranda, Roberto Donoso-Barros