By Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki
Animal mobile expertise is a becoming self-discipline of mobile biology which goals not just to appreciate buildings, capabilities and behaviors of differentiated animal cells but in addition to check their skills for use in commercial and clinical reasons. The target of animal mobile expertise comprises accomplishments of clonal enlargement of differentiated cells with invaluable skill, optimization in their tradition stipulations, modulation in their skill to supply medically and pharmaceutically, vital proteins, and the applying of animal cells to gene treatment and synthetic organs. This quantity offers the readers a whole evaluation of current cutting-edge in Japan. The lawsuits should be worthwhile for cellphone biologists, biochemists, molecular biologists, immunologists, biochemical engineers and different disciplines with regards to animal telephone tradition, operating in both educational environments or in biotechnology and pharmaceutical industries.
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Extra resources for Animal Cell Technology: Challenges for the 21st Century
3 Observation of cross section of adhesive CHO cells under osmotic pressures of 300 and 450 mOsm/kg by AFM. Key words: Osmotic pressure, Cell morphology, Suspension culture, Adhesion culture, CHO cells References J. Lin, M. Takagi, I. Qu, P. Gao, andT. Yoshida (1998) Improvement of monoclonal antibody production by gradual increase in osmotic pressure. Cytotechnology, in press. J. Lin, M. Takagi, Y. Qu, P. Gao andT. Yoshida(1998) Metabolic flux change in hybridoma cells under high osmotic pressure.
This page intentionally left blank. W. Y. W. K. S. H. G. H. Y. PARK1 1Biological Production Unit; Central Research Center, Korea Green Cross Corpration, 22 7 Kaigal-Xi, Kiheung-Eup, Yongin-Si, Kyunggi-Do, 449-900, Korea 2DepartmentofAgricultural Chemistry, Korea University, Seoul 136-701, Korea We cultivated the hybridoma producing vWF antibody in a 10L bioreactor in order to investigate the characteristics of two different cultures, intermittent and continuous bleeding. To compare with bleeding culture non-bleeding culture was also performed.
Fleischaker, R. (1987) Microcarrier cell culture. ), pp. 59-19, Hanser, New York. A. F. (1992) Development ofthe optimal inoculation conditions for microcarrier cultures. Biotechnology and Bioengineering 39,305-3 13. F. ) , pp. 105-138, Academic Press, New York. B. (1988) Overview of cell culture systems and their scale-up in Animal cell biotechnology 3 (Spier, RE. ), pp. 179-220, Academic Press, London. B. and Thornton, B. (1982) Use of microcarrier culture for the production of herpes simplex virus (type 2) in MRC-5 cells.
Animal Cell Technology: Challenges for the 21st Century by Kouji Ikura, Masaya Nagao, Seiji Masuda, Ryuzo Sasaki